CV – D’Oosterlynck André

Surname: D’OOSTERLYNCK
First name: André
E-mail: andre@jojoba.be
Date of birth: 28-06-1931
Place of birth: Shaba, Lumbumbashi, 11°30 South / 27°30 East
Nationality: Belgium (Flemish)
Languages: Dutch mother tongue, French very good, English very good, Spanish very good, German good
Military 1952 – 1953 21 months Paramedics service

1) STUDIES
1948 – 1951 Chemistry (Mineral & Organic); elemental notions of chemistry (Ghent, English Club)

1954 – 1957 Textile (Weaving & Treatment); foreman, (Ghent, Textile Institute)

1958 – 1961 Electro-techniques; Technical Engineer (Ghent, Industrial College)

1978 Obtained a degree of yacht navigator at the institute of Sea-navigation in Ostend (Belgium).

2) CAREER

1948 – 1952 Messenger-boy, sample preparator (Shell, Petrochemy)

1953 – 1958 Laboratory Technician

1958 – 1973 Senior Chemist

1974 – 1975 R&D demoulding of ytong cellular concrete

1976 – 1996 Manager of Aurelia books (Ltd.)

1988 – 2004 Labo d’O N.V. (President + Director R&D)

2004 – today D’Oosterlynck R&D

3) REALISATIONS

1957 Formulating an unmeltable lubricating grease (Sovarex)

1958 Liquid-liquid extracts of Mercaptan-Sulphur (SHELL) followed by an original method to separate sulphur organic compounds with air as reagents , due to governmental rules against environmental pollution (see below 1970).

1959 – 1961 Author of following patents:

New procedure for making LVI – oils’ (SHELL) Hot-treatment separation of unsaponifiable oils in carboxylic naftenates-emulsions’ (SHELL) Development and production of an electronic ignition (AD32) device for gas burningengines (granted with a golden plate on the ‘inventions exhibition’ Brussels, 1963).
1968 As a physico-chemical specialist in the steam boiler technology, developed a new method for cleaning and controlling CaO-MgO-SiO₂ deposits in the boiler. Although never patented, this invention was recognised by the Shell-engineers for its exceptional efficiency and economic value.
1970 Pioneer in using ammonia as a catalyst and air as a reagent to remove corrosive sulphur compounds (H₂S and S–mercaptans) from straight run produced gasoline (SHELL).
1972 Starting from an oil-residue, I upgraded the formulation of an ‘expansion seal’ used in concrete building (SHELL).
1973 I left the SHELL for personal reasons, especially because of the aggressive-amoral policy of my superiors.
1975 Developed as an independent consultant, a new product for demoulding the so called ‘Ytong’ (cellular) concrete blocks for Masonry Building (CBR-holding Société Générale de Belgique). I became a specialist in surface-treating steel moulds. I stopped this business due to the aggressive unlawful policy of my so called competitors (SHELL, Soc. Gen.de Belgique).

1976 I became manager of a scientific editorial (Aurelia books) on the Flemish market with paramedic books and videos for educational purposes. I sold this business (copy-right and distribution) in 1997 (Kluwer-editorials).
1987 I was introduced to the jojoba industry through – an investor – Mr. Edwin Verhulst (President EMEC, air conditioning & heating systems). My scientific research started with the lecture of the existing scientific publications concerning jojoba. I received from Prof. Ralph Price (University of Tucson, Arizona) a few grams of pure dimethyl Simmondsin. Testing the biological activity revealed its non-toxicity, in contradiction with 174 previous scientific publications (mainly USA publications).
1988 I personally tried first to achieve the highest added value of jojoba oil in practice, resulting in an important number of cosmetic formulations resulting in a Labo d’O (jojoba oil based ‘cosmeceutics’).

I became member of the jojoba research team of the Catholic university Leuven (KUL, Belgium). Providing the base materials (Simmondsins) for this study, the KUL realised a number of experiments, proving the anorexic property of dimethyl Simmondsin.
1991 De Heilzame kracht van jojoba (1991) André d’Oosterlynck & Ruud Verhagen. Madal bal BV. Den Haag 47 p
1993 It became clear that, due to premature publications of KUL, the originality of this discovery was lost (Re.: Marnix Cokelaere; KUL).

1994 Due to this misunderstanding I conducted my personal research program concerning the whole jojoba plant, at the University of Ghent (UG, Belgium), Faculty of Plant Biochemistry (Prof. Luc De Cooman and Phd. Dr. Evert Everaert), where I studied the plant biochemistry (identification of the so called anti-trypsin-factors and the amazing inhibitory activity of some molecules on micro-organisms, such as Staphylococcus Aureus).

1996 I continued working as a researcher at the University of Ghent (UG, Belgium), Faculty of Organic Chemistry, separation techniques, Prof. P. Sandra.
Due to incomprehensible phenomena observed in our animalarium, we started to investigate the side effects of the Simmondsins as a food supplement made by formulating refined jojoba flour as a meal.
1997, September 30th I was granted an Europa-USA-Israël patent: Method for separating the toxic resinous fraction from prepared whole jojoba seeds or jojoba seed press-cake (United States patent n° 5,672,371).
1999 Investigation by the microbiological institute (University of Ghent, UG, Belgium) on the biological activity of some polyester products (anti-biotic capacities against various bacterial strains).
2002 I sponsored a study at the institute for Experimental Oncology (UG-Prof. M. Mareel). Using Simmondsins in the CAM-assay, it became very obvious that these products were angiogenesis inhibitors.
With this elemental information Stefaan Raes and I, we went to PhD. P. Koolwijk & PhD.P. Quax both researchers at TNO-PG Institute Leiden, (the Netherlands).
Mid 2002, we received from TNO confirming results concerning the angiogenesis inhibiting properties of the different Simmondsins. We took a patent – pending, dated july 8th 2002 titled: ‘Use of Simmondsins as an angiogenetic inhibitor.
2003, July 7th This claim was declared ‘original’ later on by the International Bureau in The Hague, the Netherlands. I started  the PCT-procedure in April 2003 and this document was filed on July 7th 2003 under International Patent  Application
PCT/EP03/07270 .

2005 I started, with new partners, the production of cosmeceutics based on jojoba oil (Labo d’O)
2008, June 17th   US patent publication 7387999, granted on June 17th 2008,
“THE USE OF SIMMONDSIN AS AN ANGIOGENESIS INHIBITOR” by D’Oosterlynck/Raes

CV – Raes Stefaan

Surname : Raes
First name : Stefaan
Address : Oude Gaverse Steenweg 82 A
9820 Merelbeke
Belgium
Phone : +32/(0)9-232.19.57
Fax : +32/(0)9-211.11.02
Mobile : +32/474.99.06.77
Email : stefaanraes@jojoba.be
Date of birth : Gent, June 22^th 1967
Nationality : Belgium (Flemish)
Civil status : Living together
Military service : Accomplished in 1991-1992 (General staff, Weiden, Germany)
Languages :

• Dutch : Mother tongue

• English : Very good

• French : Very good

• German : Good

Educations :

• 1979-1985 : Secondary school, Sciences St-Jan-Berchmanscollege Heiveldstraat 117 St-Amandsberg (Gent) 9040, Belgium

• 1985-1991 : University degree in Sciences (Animal Biotechnology) State University of Gent, Faculty of Exact Sciences K.L. Ledeganckstraat 35, Gent 9000, Belgium

(2 Distinctions)

Thesis :‘Study on the stability of transformant-DNA in Trichoderma harzianum’ (Promotor Prof. Dr. M. Van Montagu)

• 1991-1993 : University degree in Molecular Biology

State University of Gent, Faculty of Exact Sciences

K.L. Ledeganckstraat 35, Gent 9000, Belgium

(Distinction)

Thesis :‘Subcellular localization of flavonoid-enzymes in Petunia hybrida’ (Promotor Prof. Dr. M. Van Montagu)

• 03/1994-08/1994 : Training course in analogue electronics

V.D.A.B., Industrieweg 50, Wondelgem 9032, Belgium

• 1996 : Course in Immunology of Domestic Animals

State University of Gent, Fac. of Veterinary Sciences

Salisburylaan 133, Merelbeke 9820

Prof. Dr. B. Goddeeris (Distinction)

Working experience :

1. • From January 1994 till august 1994, I’ve worked voluntarily as researcher on the lab. of Plant Biochemistry, Fac. of exact Sciences, State University of Gent, K.L. Ledeganckstraat 35, Gent 9000, Belgium (Prof. Dr. De Cooman). I’ve studied the nod-metabolites involved in the interaction between Leguminosa and Rhizobium. Therefore, I’ve optimized the bio-synthesis of radio-active labeled flavonoids using Petunia hybrida enzyme-extracts.

   • From September 1994 till august 1998, I was employed at the lab of Parasitology, Fac. of Veterinary Medicine, State University of Gent, Salisburylaan 133, 9820 Merelbeke, Belgium (Prof. Dr. J. Vercruysse) on a project sponsored by the Flemish government. The research was focused on the development of a vaccine against gastro-intestinal nematodes in ruminants. Candidate protective antigens were isolated, purified and evaluated in several vaccination trials with varying success. I assisted on the serology of a second project about sarcoptic mange in swine. Meanwhile, I also gave practical courses on microscopy of parasite protozoa to the students and I was responsible for the safety and waste collection of the department.

   • From September 1998 till August 2000 I was appointed as an assistant at the lab of Human Anatomy and Embryology, Fac. of Human Medicine, State University of Gent, Godshuizenlaan 4, Gent 9000, Belgium. Besides the practical courses of Human Osteology, my scientific research involved a study of the relationship between peroxisomes, GAP-junctions and apoptosis in a granulosa cell model.

   • From September 2000 till January 2005, I have been employed by Ventana Medical Systems, Parc d’Innovation, BP 30144, Rue G. de Kaysersberg, 67404 Illkirch (Strasbourg), France. Ventana has become the world leader in automation of immunohistochemistry and in-situ hybridization. The first 3 years I was mainly based at Strasbourg for training and assisting in the reorganization of the technical department. Besides customer visits allover Europe to solve technical and application issues, I developed numerous technical procedures, data sheets etc…

   • Since May 2007, I’m working for Perkin Elmer (Imperioastraat 8, 1930 Zaventem) as Field service engineer Chromatography (LC,GC,MS).

   • I came in contact with Mr. André d’Oosterlynck in 1994 at the lab of Plant biochemistry (State University of Gent) who introduced me to his jojoba research. I got intrigued by the conflicting literature and results concerning the application of refined, de-oiled meal of the jojoba plant, and got finally involved in this research besides my main profession. I could use the infrastructure the lab of Parasitology to conduct several feeding experiments on mice. Feeding mice with jojoba meal at a threshold dose whereby no significant weight loss was induced, led to a reversible incapability of reproduction throughout the whole experiment. This led to our conclusion that some other factors present in jojoba meal showed other unrevealed activities. In 2000, I launched the hypothesis that jojoba meal contained angiogenesis inhibiting properties, which got confirmed later on by the TNO institute of Leiden (The Netherlands) and finally resulted in our patent application.

      

Scientific output:

Publications :

• De Graaf D.C., Bergen P., Moens L., De Marez T.M., Raes S., Blaxter M.L. and Vercruysse J. (1996) Isolation, Characterization and Immunolocalization of a globin-like antigen from Ostertagia ostertagi adults. Parasitology : 113, 63-69

• Hollanders W., Vercruysse J., Raes S. and Bornstein S. (1997) Evaluation of an Enzyme-Linked Immunosorbent Assay (ELISA) for the serological diagnosis of sarcoptic mange in swine. Veterinary Parasitology 69, 117-123

• Van Aken D., Vercruysse J., Dargantes A.P., Lagapa J.T. Raes S. and Shaw D.J. (1997) Pathophysiological aspects of Mecistocirrus digitatus (Nematoda : Trichostrongylidae) infection in calves. Veterinary Parasitology 69, 255-263

• Farioli-Vecchioli S., Raes S., Espeel M., D’Herde K., Roels F. (2000) Inverse expression of peroxisomes and connexin-43 in the granulose cells of the quail follicle. J. Histochem. Cytochem. 48 (2), 167-177

• Raes S., Vervaet P. and d’Oosterlynck A. Effects of jojoba meal on the fertility of mice (Not published yet)

Congress Communications :

• Raes S., de Graaf D.C. and Vercruysse J. (1996) Purification and characterization of the native hemoglobin from Ostertagia ostertagi. Proceedings : Spring meeting of the British Society of Parasitology 146, p 49. University of Wales, Bangor, England, 1-3 april 1996.

• Raes S., Claerebout E. and Vercruysse J. (1998) Study of the local IgA response of calves infected with the abomasal nematode Ostertagia ostertagi. Proceedings of the 1998 meeting of the Belgian Society for Parasitology, p 11-14. University of Leuven, Belgium, 20 february 1998

• Raes S., Claerebout E. and Vercruysse J. (1998) Abomasal IgA responses to Ostertagia ostertagi infections in cattle. Proceedings : Spring meeting of the British Society of Parasitology 112, p 43. University of Exeter, 6-8 april 1998.

• Farioli-Vecchioli S., Raes S., Roels F. and D’Herde K. (1999) Inversed expression of peroxisomes and GAP-junctions in the granulose of the quail follicle. Proceedings of the 12^th European study group on Lysosomal Diseases workshop. Abstract 36. Vidago, Portugal, 23-26 september 1999.

Abstracts/Posters :

• Farioli-Vecchioli S., Raes S., Espeel M., D’Herde K and Roels F. (1999) Is regional variation in peroxisomal expression in avian granulose cell sheets related to GAP-junctional communication ? Autumn meeting ‘ Developmental Neurobiology’ of the Belgian Society for Cell Biology, 17^th October 1998, Namur, Belgium. Cell Biol. Int. 23 (2), 142

• Depreter M., Walker T., Raes S., De Smet K., Espeel M., Rogiers V. and Roels F. (2000) Hepatocyte polarity and peroxisome differentiation in three culture types and during development. Spring meeting ‘Apoptosis’ of the Belgian Society for Cell Biology, 6^th march 1999, Gent, Belgium. Cell Biol. Int.

• Raes S., D’Herde K. and Roels F. (2000) Effect of blocking GAP-Junctional communication on peroxisomal expression and apoptosis. .Spring meeting ‘Apoptosis’ of the Belgian Society for Cell Biology, 6^th march 1999, Gent, Belgium. Cell Biol. Int.

• Depreter M., Walker T., Desmet K., De Prest B., Raes S., Roels F. (1999). Localization of ATP/ADP and AMP hydrolyzing enzyme activities as a tool to assess hepatocyte polarity. Proceedings 2^nd Int. Workshop on ecto-ATPases and related ectonucleotidases (Shaker Publ. BV., Maastricht)

Patents :

• Co-author on patentPCT/EP03/07270, titled : ‘Simmondsin for use as an Angiogenesis Inhibitor’, filed on 8^th july 2003, US patent publication 7,387,999 granted on June 17th,2008 .

Stages & Workshops :

• Miniaturizing liquid chromatography vs. capillary electrophoresis. University of Gent, Fac. Pharmaceutical Sciences, 23 may 1996.

• Stage at the University of Edinburgh (Scotland), Ashworth laboratories, division of Biological Sciences (Dr. M. Blaxter) from 22/07/96 till 02/08/96. Cloning an Ostertagia ostertagi hemoglobin gen.

• Workshop ‘Molecular Biology and Recombinant DNA technology’ from June 16^th till June 27^th 1997 at the University of Utrecht (The Netherlands)

Additional information : 

• Driving license Cat. B since 1985

• I was an active presidium member of the G.B.K student’s organization (Biological Circle of Gent) from 1987 till 1991

• Computer skills : Installation of hardware & software, knowledge of using MS-DOS, Windows, Office,  Fortran77, Internet…

• Since electronics and mechanics are one of my favorite hobbies, I have a quite good experience in maintenance and repair of all kind of apparatus, including laboratory equipment. I developed this skill more intensively at my last jobs (Ventana Medical Systems and Perkin Elmer)

• During my collaboration with Labo D’O, I got aware of the great importance in the relationship between food & health, and the remarkable power of some alternative medicine techniques which I try to understand and clarify using scientific methodology.